Objective: Interlocking nails to stabilize humeral shaft fractures are attractive due to the minimal invasive operative technique. Recently attempts have been made to use plates in a minimally invasive technique (MIPO) in the humerus. Both, interlocking nails and MIPO need reliable anatomical landmarks to avoid iatrogenic nerve damage. The purpose of this study is to provide a map of safety zones at the humerus to minimize the risk of iatrogenic nerve damage. Material and technique: 22 upper extremities from donors of the anatomical institute were investigated to identify routes and positions of the axillary and the radial nerve. A nail supported targeting device was designed to define the position of nerve structures at the humerus. All hits were recorded in a table based on their distance to the humeral head. The data pool was used to define areas of safety at the posterior, lateral anterior aspect of the humerus. Results: Hits of the axillary nerve were found in a 40 mm broad sector, located 30 to 40 mm distal to the tip of the greater tubercle. Between the horizontal course of the axillary nerve and the descending course of the radial nerve a broad triangular safety zone was identified. On the dorsal aspect of the humerus, this safe area was small with a vertical extension of 30 mm, located 80 mm distal to the tip of the humeral head. The axillary nerve takes a horizontal route. The distance of the safety area to the humeral head keeps being 80 mm distal. The radial nerve has a descending route from dorsal to ventral and the safety zone between both nerves is thus getting broader. On the lateral aspect of the humerus, it measures 80 mm and anterior 130 mm. Variation of the nerve position is high, but most hits of the axillary and radial nerve were found in a 20 mm broad sector for both nerves. Conclusions: The data provide a map of nerve position and safety zones that help to place orthopedic devices (interlocking screws, plates) in the humerus using minimally invasive techniques in a favorable position minimizing the risk of iatrogenic nerve damage.